Journal: Clinical and Experimental Immunology

Article Title: Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

doi: 10.1111/cei.13430

Figure Lengend Snippet: Detection of serum IgG antibody titers to periodontal pathogens in favorable and unfavorable groups after stroke

Article Snippet: Sonicated preparations of the following periodontal pathogens were used as bacterial antigens in the present study: Porphyromonas gingivalis ATCC33277 (fimA type I), HW24D1 (fimA type II), 6/26 (fimA type III), W83 (fimA type IV) and HNA99 (fimA type V); Aggregatibacter actinomycetemcomitans ATCC29523 (serotype a), Y4 (serotype b) and AUNY67 (serotype c); Prevotella intermedia ATCC26511, P. nigrescens ATCC33563; Fusobacterium nucleatum ATCC25586 (subspecies nucleatum ) and ATCC 10953 (subspecies polymorphum ); Treponema denticola ATCC35405; Tannerella forsythensis ATCC43037; Campylobacter rectus ATCC33238; and Eikenella corrodens ATCC23834.

Techniques:

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Mechanisms of reduced susceptibility and genotypic prediction of antibiotic resistance in Prevotella isolated from cystic fibrosis (CF) and non-CF patients

doi: 10.1093/jac/dku192

Figure Lengend Snippet: Prevalence of resistance genes (cfxA-type gene, ermF and tetQ) in each of the Prevotella groups.

Article Snippet: Detection of species-specific RND-type efflux pumps Protein sequences of RND efflux pumps in P. aeruginosa PAO1 (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY-OprM) and B. fragilis ATCC 25285 (BmeABC1–8) were searched for homologies within five Prevotella genomes ( Prevotella denticola F0289, Prevotella nigrescens ATCC 33563, Prevotella melaninogenica ATCC 25845, Prevotella histicola F0411 and Prevotella salivae DSM 15606) using protein BLAST.

Techniques:

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Mechanisms of reduced susceptibility and genotypic prediction of antibiotic resistance in Prevotella isolated from cystic fibrosis (CF) and non-CF patients

doi: 10.1093/jac/dku192

Figure Lengend Snippet: Antibiotic susceptibility (MICs) of Prevotella isolates (n = 25) in the absence or presence of the EPI Phe-Arg β-naphthylamide dihydrochloride. In the box and whisker plots, the top and bottom boundaries of each box indicate the 75th and 25th quartile values, respectively, with the line inside the box representing the median (50th quartile). The ends of the whiskers indicate the range. Any isolates recorded as having an MIC greater than the maximum concentration (>256 mg/L) or lower than the minimum concentration (<0.016 mg/L) on the Etest® strip are shown as double the maximum concentration or half the lower concentration, respectively.

Article Snippet: Detection of species-specific RND-type efflux pumps Protein sequences of RND efflux pumps in P. aeruginosa PAO1 (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY-OprM) and B. fragilis ATCC 25285 (BmeABC1–8) were searched for homologies within five Prevotella genomes ( Prevotella denticola F0289, Prevotella nigrescens ATCC 33563, Prevotella melaninogenica ATCC 25845, Prevotella histicola F0411 and Prevotella salivae DSM 15606) using protein BLAST.

Techniques: Whisker Assay, Concentration Assay, Stripping Membranes

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Mechanisms of reduced susceptibility and genotypic prediction of antibiotic resistance in Prevotella isolated from cystic fibrosis (CF) and non-CF patients

doi: 10.1093/jac/dku192

Figure Lengend Snippet: Amino acid sequence analysis of 20 clinical Prevotella isolates and a type strain, P. pallens NCTC 13042, compared with CfxA from B. vulgatus {"type":"entrez-protein","attrs":{"text":"AAB17891","term_id":"1079658","term_text":"AAB17891"}} AAB17891 23

Article Snippet: Detection of species-specific RND-type efflux pumps Protein sequences of RND efflux pumps in P. aeruginosa PAO1 (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY-OprM) and B. fragilis ATCC 25285 (BmeABC1–8) were searched for homologies within five Prevotella genomes ( Prevotella denticola F0289, Prevotella nigrescens ATCC 33563, Prevotella melaninogenica ATCC 25845, Prevotella histicola F0411 and Prevotella salivae DSM 15606) using protein BLAST.

Techniques: Sequencing, Control

Journal: Aging Cell

Article Title: Loss of tenomodulin expression is a risk factor for age‐related intervertebral disc degeneration

doi: 10.1111/acel.13091

Figure Lengend Snippet: Double knockout for Tnmd and Chm1 lead to accelerated IVD degeneration coupled with ectopic bone formation. (a and b) Immunofluorescence analysis of Chm1 expression shows Chm1 major localization in the NP as well as lower protein levels in Tnmd −/− than WT IVD at 6 months (two‐tailed nonparametric Mann–Whitney test; n = 5 animals). (c) Western blotting confirmed the downregulation of Chm1 protein in Tnmd −/− compared to WT IVD ( n = 3 animals). (d‐f) H&E and safranin O staining reveal advanced IVD degeneration and ectopic bone formation in Tnmd −/− Chm1 −/− mice, and histological grading shows significantly worsened scores at 18 months in Tnmd −/− Chm1 −/− mice compared to Tnmd −/− and WT mice (one‐way ANOVA was followed by Bonferroni post hoc correction, n = 3 animals). (g) Immunofluorescence analysis for CD31 demonstrate that Tnmd −/− Chm1 −/− OAF contain many capillary‐like structures ( n = 3 animals). (h) Immunofluorescence analysis for F4/80 shows many macrophages were distributed in and around the NP of Tnmd −/− Chm1 −/− mice ( n = 3 animals). (i) Multiple sites of ectopic ossifications in Tnmd −/− Chm1 −/− IVD were observed by carrying out Opn immunostaining ( n = 3 animals). * p < .05; ** p < .01; *** p < .001. AF, annulus fibrous; EP, endplate; H&E, hematoxylin–eosin; IAF, inner annulus fibrous; IVD, intervertebral disc; NP, nucleus pulposus; OAF, outer annulus fibrous; WT, wild‐type; black arrows, chondrocyte‐like cells; white arrows, ectopic ossifications; white dotted line, OAF‐IAF boundary. Scale bar: 200 μm

Article Snippet: After washing and blocking with 2% bovine serum albumin/PBS (Sigma‐Aldrich), primary antibodies against Acan (Abcam, 1:100, ab36861), CD31 (Abcam, 1:50, ab28364), Chm1 (Santa Cruz Biotechnology, 1:200, sc‐33563), Col I (Abcam, 1:50, ab34710), Col X (Abcam, 1:50, ab58632), Fmod (Abcam, 1:200, ab81443), Fn (Abcam, 1:50, ab2413), F4/80 (Abcam, 1:100, ab100790), Lum (Abcam, 1:50, ab168348), MMP‐3 (Novus Biologicals, 1:100, NB110‐57221), MMP‐9 (Millipore, 1:100, AB19016), Opn (Abcam, 1:200, ab8448), PCNA (Invitrogen, 1:100, 13‐3900), p53 (Abcam, 1:100, ab61241), p65 (Abcam, 1:1000, ab16502), Runx2 (Abcam, 1:200, ab102711), Sox9 (Abcam, 1:400, ab3697), and Tnmd (Metabion, PAB 201603‐00002, 1:100) were applied overnight at 4°C.

Techniques: Double Knockout, Immunofluorescence, Expressing, Two Tailed Test, MANN-WHITNEY, Western Blot, Staining, Immunostaining